Cutthroat Trout chromosome-level genome assembly

Authors: R. Evans, Kevin Rogers, Dennis Shiozawa

Year: 2022

Abstract

A chromosome-level genome assembly for Oncorhynchus clarkii stomias (Greenback Cutthroat Trout) was produced using Illumina, Pacific Biosciences, Hi-C, and transcriptome data from a Bear Creek, Colorado population male. The assembly spans 2.3 Gb with a contig N50 of 11.2 Mb and 97% of the genome assigned to 33 chromosomes. The observed high rate of duplication for eukaryotic universal single-copy orthologs (BUSCO), 46%, is consistent with the autotetraploid origin of the Oncorhynchus genome. A reference genome for Cutthroat Trout allows for ready implementation of next generation sequencing based, conservation directed analyses of individuals and populations in this enigmatic species. FRIDAY MORNING, NOVEMBER 18 –